產(chǎn)品信息

產(chǎn)品簡(jiǎn)介

     The NxSeq? UltraLow DNA Library Kit supplies the buffers and   enzymes needed to make high efficiency DNA fragment libraries for whole   genome next generation sequencing on Illumina sequencers. This kit is   optimized for the production of DNA fragment libraries starting with 50 pg to   75 ng of sheared/fragmented DNA. This kit may be used in other applications   such as FFPE, ChIP and exome sequencing where PCR amplified libraries are   required, but it has not been tested extensively in these applications. Note, the number of PCR cycles required for amplification is dependent on the starting amount of the ultralow input sheared DNA. Also, the number of observed PCR duplicates sequenced is dependent on sample complexity, number   of multiplexed samples, and the model of Illumina sequencer used.
      NxSeq?UltraLowDNA文庫(kù)試劑盒提供制備高效DNA片段文庫(kù)所需的緩沖液和酶，用于Illumina測(cè)序儀的全基因組二代測(cè)序。 該試劑盒針對(duì)DNA片段文庫(kù)的生產(chǎn)進(jìn)行了優(yōu)化，從50 pg至75 ng的剪切/片段化DNA開(kāi)始。 該試劑盒可用于其他應(yīng)用，如FFPE，ChIP和外顯子組測(cè)序，其中需要PCR擴(kuò)增文庫(kù)，但尚未在這些應(yīng)用中進(jìn)行廣泛測(cè)試。 注意，擴(kuò)增所需的PCR循環(huán)數(shù)取決于超低輸入剪切DNA的起始量。 此外，測(cè)序的觀(guān)察到的PCR重復(fù)序列的數(shù)量取決于樣品復(fù)雜性，多重樣品的數(shù)量和所用Illumina測(cè)序儀的模型。
This User Manual couples the NxSeq? UltraLow DNA Library Kit, 12   Reactions with the NxSeq? Single Indexing Kits for lower throughput   sequencing experiments. For higher throughput sequencing experiments (e.g. 96   library preps in a 96 well plate), we recommend using the NxSeq? UltraLow DNA   Library Kit, 96 Reactions (Cat. No. 15096-1) with the NxSeq? HT Dual Indexing   Kit (Cat. No. 15300-1). If you prefer to use dual indices with the NxSeq?   UltraLow DNA Library Kit, 12 Reactions, please follow the amplification protocol outlined in the NxSeq? UltraLow DNA Library Kit, 96 Reactions User   Manual and adapt it to the single tube reaction format.
     本用戶(hù)手冊(cè)將NxSeq?UltraLowDNA文庫(kù)試劑盒，12種反應(yīng)與NxSeq?單一index試劑盒結(jié)合，進(jìn)行低通量測(cè)序?qū)嶒?yàn)。 對(duì)于更高通量的測(cè)序?qū)嶒?yàn)（例如96孔板中的96個(gè)文庫(kù)制備），我們建議使用NxSeq?UltraLowDNA文庫(kù)試劑盒，96反應(yīng)（貨號(hào) 15096-1）和NxSeq?HTindex試劑盒（貨號(hào) 15300-1）。如果您更喜歡使用NxSeq?UltraLowDNA Library Kit，12 Reactions進(jìn)行雙index，請(qǐng)遵循NxSeq?UltraLowDNA Library Kit，96 Reactions User Manual中列出的擴(kuò)增方案，并使其適應(yīng)單管反應(yīng)形式。
優(yōu)點(diǎn)：
? High Quality Data: High efficiency adaptor ligation produces complex libraries that yield improved sequencing depth uniformity and better   coverage with fewer zero coverage regions
- 高質(zhì)量數(shù)據(jù)：高效率的接頭連接產(chǎn)生復(fù)雜的文庫(kù)，可以提供更好的測(cè)序深度均勻性和更好的覆蓋率，減少零覆蓋區(qū)域
? Sensitive: Construct DNA fragment libraries from as little as 50pg to as much 75 ng of sheared/fragmented DNA
- 靈敏：構(gòu)建DNA片段文庫(kù)，從低至50 pg到75 ng剪切/片段化DNA
? Minimal Bias: Robust, uniform PCR amplification improves coverage uniformity when working with low input amounts of genomic DNA
- 最小偏差：當(dāng)使用低輸入量的基因組DNA時(shí)，穩(wěn)健，均勻的PCR擴(kuò)增可提高覆蓋均勻性
? Flexible:Extensively tested in de novo whole genome sequencing or resequencing, but compatible with other applications such as exome-seq,ChIP-seq and FFPE DNA samples.
- 靈活：在全新的全基因組測(cè)序或重新測(cè)序中進(jìn)行了廣泛測(cè)試，但與exome-seq，ChIP-seq和FFPE DNA樣品等其他應(yīng)用也兼容。
? Fast: 3 hour protocol gets your samples on the sequencer quicker
- 快速：3小時(shí)操作即可，可以更快地進(jìn)行測(cè)序
?High Value: Cost-effective library and indexing kits which produce excellent sequencing results 
? 高價(jià)值：經(jīng)濟(jì)高效的文庫(kù)和index試劑盒，可產(chǎn)生出色的測(cè)序結(jié)果
數(shù)據(jù)：

Figure 3. Typical Bioanalyzer trace for library generated using input gDNA sheared mechanically   to 350 bp.

 Figure 4. Typical Bioanalyzer traces for final libraries generated using input gDNA sheared enzymatically.

組成成分：
? Enzyme Mix：-20°C
? 2X Buffer ：-20°C
? Ligase：-20°C
? Elution Buffer：-20°C
? 2X PCR Master Mix：-20°C